ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (9): 1425-1431.doi: 10.11843/j.issn.0366-6964.2013.09.013

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The Effect of Soybean Trypsin Inhibitor on Generation of Oxygen Free Radical in vivo at Different Growth Stages of Mice

GU Chun-mei1, HAN Ling-ling1, ZHAO Lin-lin1, QU Hong-sheng1,SONG Xin-xiu1, PAN Shu1, QIN Gui-xin2*   

  1. (1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118，China; 2. College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, China)
  • Received:2012-12-24 Online:2013-09-23 Published:2013-09-23

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Abstract:

 To study the effect of STI on the generation of oxygen free radical in vivo at different growth stages of mice, 180 clean and male KM mice were selected and randomly divided into three groups. The first group as a control group was fed control diet, the second group(STI group) was fed control diet containing STI，the third group(VC group) was fed STI diet supplemented with vitamin C. After mice being fed 1, 2, 3, 4 and 5 week, serum and pancreas samples were collected to measure oxidative and antioxidative parameters. The results showed that, compared with the control group, the oxidant parameters significantly increased and the antioxidant parameters significantly decreased in serum and pancreas of STI group (P<0.05). In the whole growth periods, MDA content increased in the first two weeks, and achieved the maximum in the third week, then dropped. The ability of anti-superoxide anion firstly increased, then dropped, again increased and decreased. The ability of inhibiting hydroxy radical slowly increased. After VC being supplemented, the level of oxidation decreased, but its value was still significantly higher than that of control group (P<0.05). The activities of T-SOD in serum and pancreas dropped in the first two weeks, achieved the minimum in the third week, then increased. The activities of CAT in pancreas slowly increased . After VC being supplemented, the ability of antioxidant defenses in serum and pancreas were enhanced, but still lower than that of control group (P<0.05). The present study indicates that the effect of STI on free radical level in vivo of mice is related to growth periods, and this effect is the most significant in the third week. In addition, VC can interfere with the role of STI on the organism and effectively improve the status of oxidative stress.

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